phosphonrf2 ser40 antibody Search Results


phosphonrf2 ser40 antibody  (MedChemExpress)
94
MedChemExpress phosphonrf2 ser40 antibody
Phosphonrf2 Ser40 Antibody, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphonrf2+ser40+antibody/Phospho-Nrf2+(Ser40)+Antibody/pm40240513-198-0-7
Average 94 stars, based on 1 article reviews
phosphonrf2 ser40 antibody - by Bioz Stars, 2026-09
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phospho histone h2a x ser139  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc phospho histone h2a x ser139
Fig. 3. Plasma-Activated Media (PAM) induces apoptosis in High-Grade Serous Ovarian Cancer Cell (HGSOC) cell lines. (A) CAOV3 cells were pre-treated with pan-caspase inhibitor Q-VD-OPh (QVD) (50 μM) for 2 h prior to addition of control (Ctl), argon-treated (Argon) or high- dose PAM. The cell numbers were quantified by MTS after 24 h. (B) CAOV3 cells were pre-treated with GEE for 2 h prior to addition of control, argon-treated or high-dose PAM. The cell numbers were quantified using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2- (4-sulfophenyl)-2H-tetrazolium (MTS) viability assay after 24 h. (C) CAOV3 cells were pre-treated with ferroptosis inhibitor Ferrostatin-1 (Ferr- 1) (2 μM) for 1 h prior to addition of media control, argon-treated media control, high-dose PAM or pro-ferroptotic agent RSL-3 (2 μM). The cell numbers for A–C were quantified by MTS 24 h after treatment. MTS results are normalised to % media control and represented as mean Standard Deviation for three independent experiments (n = 3). Significance was determined by one-way ANOVA with multiple comparisons (GRAPHPAD, PRISM v9). ** = P < 0.01, **** = P < 0.0001, ns, not significant. (D) CAOV3 cells were treated with media control, argon control, medium- or high-dose PAM for the indicated times. Cleared lysate samples were subjected to SDS/PAGE and immunoblotting for: oxidative stress marker p-NRF2 (Ser40); DNA damage marker <t>p-H2A.X</t> <t>(Ser139);</t> caspase-activation marker PARP-1 (full length 116 kDa, cleaved 89 kDa); and loading control Tubulin. Blotting results are representative of three independent experiments (n = 3).
Phospho Histone H2a X Ser139, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphonrf2+ser40+antibody/Phospho-Histone+H2A%2EX+(Ser139)+Rabbit+mAb/pm39626867-102-15-18
Average 99 stars, based on 1 article reviews
phospho histone h2a x ser139 - by Bioz Stars, 2026-09
99/100 stars
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Fig. 3. Plasma-Activated Media (PAM) induces apoptosis in High-Grade Serous Ovarian Cancer Cell (HGSOC) cell lines. (A) CAOV3 cells were pre-treated with pan-caspase inhibitor Q-VD-OPh (QVD) (50 μM) for 2 h prior to addition of control (Ctl), argon-treated (Argon) or high- dose PAM. The cell numbers were quantified by MTS after 24 h. (B) CAOV3 cells were pre-treated with GEE for 2 h prior to addition of control, argon-treated or high-dose PAM. The cell numbers were quantified using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2- (4-sulfophenyl)-2H-tetrazolium (MTS) viability assay after 24 h. (C) CAOV3 cells were pre-treated with ferroptosis inhibitor Ferrostatin-1 (Ferr- 1) (2 μM) for 1 h prior to addition of media control, argon-treated media control, high-dose PAM or pro-ferroptotic agent RSL-3 (2 μM). The cell numbers for A–C were quantified by MTS 24 h after treatment. MTS results are normalised to % media control and represented as mean Standard Deviation for three independent experiments (n = 3). Significance was determined by one-way ANOVA with multiple comparisons (GRAPHPAD, PRISM v9). ** = P < 0.01, **** = P < 0.0001, ns, not significant. (D) CAOV3 cells were treated with media control, argon control, medium- or high-dose PAM for the indicated times. Cleared lysate samples were subjected to SDS/PAGE and immunoblotting for: oxidative stress marker p-NRF2 (Ser40); DNA damage marker p-H2A.X (Ser139); caspase-activation marker PARP-1 (full length 116 kDa, cleaved 89 kDa); and loading control Tubulin. Blotting results are representative of three independent experiments (n = 3).

Journal: Molecular oncology

Article Title: Plasma-activated media selectively induces apoptotic death via an orchestrated oxidative stress pathway in high-grade serous ovarian cancer cells.

doi: 10.1002/1878-0261.13768

Figure Lengend Snippet: Fig. 3. Plasma-Activated Media (PAM) induces apoptosis in High-Grade Serous Ovarian Cancer Cell (HGSOC) cell lines. (A) CAOV3 cells were pre-treated with pan-caspase inhibitor Q-VD-OPh (QVD) (50 μM) for 2 h prior to addition of control (Ctl), argon-treated (Argon) or high- dose PAM. The cell numbers were quantified by MTS after 24 h. (B) CAOV3 cells were pre-treated with GEE for 2 h prior to addition of control, argon-treated or high-dose PAM. The cell numbers were quantified using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2- (4-sulfophenyl)-2H-tetrazolium (MTS) viability assay after 24 h. (C) CAOV3 cells were pre-treated with ferroptosis inhibitor Ferrostatin-1 (Ferr- 1) (2 μM) for 1 h prior to addition of media control, argon-treated media control, high-dose PAM or pro-ferroptotic agent RSL-3 (2 μM). The cell numbers for A–C were quantified by MTS 24 h after treatment. MTS results are normalised to % media control and represented as mean Standard Deviation for three independent experiments (n = 3). Significance was determined by one-way ANOVA with multiple comparisons (GRAPHPAD, PRISM v9). ** = P < 0.01, **** = P < 0.0001, ns, not significant. (D) CAOV3 cells were treated with media control, argon control, medium- or high-dose PAM for the indicated times. Cleared lysate samples were subjected to SDS/PAGE and immunoblotting for: oxidative stress marker p-NRF2 (Ser40); DNA damage marker p-H2A.X (Ser139); caspase-activation marker PARP-1 (full length 116 kDa, cleaved 89 kDa); and loading control Tubulin. Blotting results are representative of three independent experiments (n = 3).

Article Snippet: PARP-1 (Cell Signaling Technology (CST), Danvers, MA, USA, #9542), PhosphoNRF2 (Ser40) (Invitrogen/Thermo Fisher Scientific, #PA5-67520), Phospho-histone H2A.X (Ser139) (CST, Danvers, MA, USA, #9718), Alpha-Tubulin (Abcam, Cambridge, UK, #ab7291).

Techniques: Clinical Proteomics, Control, Viability Assay, Standard Deviation, SDS Page, Western Blot, Marker, Activation Assay